Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13594 and 13595 are separated by 168 nucleotides13595 and 13596 are separated by 370 nucleotides13596 and 13597 are separated by 117 nucleotides13597 and 13598 are separated by 1412 nucleotides mRNA_5226: 13594 - K12472 EPS15 epidermal growth factor receptor substrate 15, at 196,713 to 201,520 13594 mRNA_5227: 13595 - K00601 E2.1.2.2 phosphoribosylglycinamide formyltransferase, at 201,689 to 202,738 13595 mRNA_5228: 13596 - HMMPfam-Mis6-PF07778, at 203,109 to 206,278 13596 mRNA_5229: 13597 - SUPERFAMILY--SSF51445, at 206,396 to 208,003 13597 mRNA_5230: 13598 - K17906 ATG2 autophagy-related protein 2, at 209,416 to 215,994 13598
Group Condition mRNA_5226 mRNA_5227 mRNA_5228 mRNA_5229 mRNA_5230
13594 13595 13596 13597 13598
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. +0.0 +0.1 -2.7
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. +0.2 -0.1 -1.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. +0.1 +0.1 -1.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.7 -0.6 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.4 -0.2 -0.6
auxotrophy Dropout Complete -0.0 N.D. -0.3 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. +0.1 +0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.1 -0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -0.1 -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. -0.1 -0.1 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.1 -0.2 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. +0.1 -0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.0 -0.1 +0.2
growth on oleic acid YNB Glucose +0.1 N.D. -0.1 -0.1 +0.1
growth on ypd YPD +0.1 N.D. +0.1 -0.0 -0.0
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.1 -0.1 +0.1
auxotrophy YNB Glucose +0.3 N.D. -0.2 -0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. -0.0 -0.0 +0.3
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.2 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. +0.1 +0.1 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.2 -0.2 +0.8
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