Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13595 and 13596 are separated by 370 nucleotides13596 and 13597 are separated by 117 nucleotides13597 and 13598 are separated by 1412 nucleotides13598 and 13599 are separated by 506 nucleotides mRNA_5227: 13595 - K00601 E2.1.2.2 phosphoribosylglycinamide formyltransferase, at 201,689 to 202,738 13595 mRNA_5228: 13596 - HMMPfam-Mis6-PF07778, at 203,109 to 206,278 13596 mRNA_5229: 13597 - SUPERFAMILY--SSF51445, at 206,396 to 208,003 13597 mRNA_5230: 13598 - K17906 ATG2 autophagy-related protein 2, at 209,416 to 215,994 13598 mRNA_5231: 13599 - Hypothetical Protein, at 216,501 to 218,178 13599
Group Condition mRNA_5227 mRNA_5228 mRNA_5229 mRNA_5230 mRNA_5231
13595 13596 13597 13598 13599
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 +0.1 -2.7 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.7 -0.6 -0.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 +0.1 -1.5 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -0.1 -1.5 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 -0.2 -0.6 -0.0
auxotrophy Dropout Complete N.D. -0.3 -0.1 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 -0.3 +0.0 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.1 -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 -0.2 +0.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 -0.2 +0.1 +0.1
growth on oleic acid YNB Glucose N.D. -0.1 -0.1 +0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +0.1 -0.0 -0.0
growth on ypd YPD N.D. +0.1 -0.0 -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.1 +0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.1 -0.1 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -0.0 +0.3 +0.2
auxotrophy YNB Glucose N.D. -0.2 -0.1 +0.4 +0.4
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.0 +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 -0.1 +0.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.2 +0.8 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.1 +0.5 +0.0
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