Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13602 and 13603 are separated by 514 nucleotides13603 and 13604 are separated by 203 nucleotides13604 and 13605 are separated by 311 nucleotides13605 and 13606 are separated by 538 nucleotides mRNA_5234: 13602 - KOG0725 Reductases with broad range of substrate specificities, at 224,900 to 226,337 13602 mRNA_5235: 13603 - KOG1178 Non-ribosomal peptide synthetase/alpha-aminoadipate reductase and related enzymes, at 226,852 to 231,804 13603 mRNA_5236: 13604 - KOG2031 Tyrosyl-DNA phosphodiesterase, at 232,008 to 233,871 13604 mRNA_5237: 13605 - K02914 RP-L34, MRPL34, rpmH large subunit ribosomal protein L34, at 234,183 to 234,602 13605 mRNA_5238: 13606 - KOG1199 Short-chain alcohol dehydrogenase/3-hydroxyacyl-CoA dehydrogenase, at 235,141 to 236,473 13606
Group Condition mRNA_5234 mRNA_5235 mRNA_5236 mRNA_5237 mRNA_5238
13602 13603 13604 13605 13606
ypd buoyancy 40 hrs Low Buoyancy +0.4 +0.0 -0.6 N.D. -0.2
growth on ypd YPD -0.8 -0.2 +0.6 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 -0.1 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 -0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine +0.6 -0.1 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.1 -0.0 N.D. +0.6
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.1 +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.2 -0.1 N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.4 -0.1 +0.1 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 -0.1 -0.2 N.D. -0.1
auxotrophy Dropout Complete +0.3 -0.0 -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 -0.4 -0.0 N.D. +0.6
growth on oleic acid YNB Glucose +0.6 +0.2 -0.3 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.5 +0.1 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 -0.1 +0.0 N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.0 +0.3 N.D. +0.5
auxotrophy YNB Glucose +0.7 -0.1 +0.3 N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.1 -0.2 N.D. +0.6
growth on ricinoleic acid YNB Ricinoleic Acid +1.7 -0.1 +0.1 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +1.2 -0.1 -0.2 N.D. +0.8
growth on ricinoleic acid YNB Glucose +1.2 +0.1 +0.4 N.D. +0.5
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