Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13603 and 13604 are separated by 203 nucleotides13604 and 13605 are separated by 311 nucleotides13605 and 13606 are separated by 538 nucleotides13606 and 13607 are separated by 150 nucleotides mRNA_5235: 13603 - KOG1178 Non-ribosomal peptide synthetase/alpha-aminoadipate reductase and related enzymes, at 226,852 to 231,804 13603 mRNA_5236: 13604 - KOG2031 Tyrosyl-DNA phosphodiesterase, at 232,008 to 233,871 13604 mRNA_5237: 13605 - K02914 RP-L34, MRPL34, rpmH large subunit ribosomal protein L34, at 234,183 to 234,602 13605 mRNA_5238: 13606 - KOG1199 Short-chain alcohol dehydrogenase/3-hydroxyacyl-CoA dehydrogenase, at 235,141 to 236,473 13606 mRNA_5239: 13607 - K10859 ALKBH2 alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, at 236,624 to 237,646 13607
Group Condition mRNA_5235 mRNA_5236 mRNA_5237 mRNA_5238 mRNA_5239
13603 13604 13605 13606 13607
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.6 N.D. -0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.2 N.D. -0.1 -0.3
growth on oleic acid YNB Oleic Acid -0.1 +0.1 N.D. -0.2 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.1 N.D. +0.0 -0.5
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.3 N.D. -0.0 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 N.D. +0.2 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 N.D. -0.1 +0.2
growth on oleic acid YNB Glucose +0.2 -0.3 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 -0.0 N.D. +0.6 -0.2
growth on ypd YPD -0.2 +0.6 N.D. +0.1 -0.5
auxotrophy Dropout Complete -0.0 -0.1 N.D. +0.3 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.1 N.D. +0.3 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.2 N.D. +0.6 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.0 N.D. +0.6 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.2 N.D. -0.3 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.0 N.D. +0.4 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.2 N.D. +0.8 -0.2
auxotrophy YNB Glucose -0.1 +0.3 N.D. +0.4 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.3 N.D. +0.5 +0.3
growth on ricinoleic acid YNB Glucose +0.1 +0.4 N.D. +0.5 +0.1
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