Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13604 and 13605 are separated by 311 nucleotides13605 and 13606 are separated by 538 nucleotides13606 and 13607 are separated by 150 nucleotides13607 and 13608 are separated by 373 nucleotides mRNA_5236: 13604 - KOG2031 Tyrosyl-DNA phosphodiesterase, at 232,008 to 233,871 13604 mRNA_5237: 13605 - K02914 RP-L34, MRPL34, rpmH large subunit ribosomal protein L34, at 234,183 to 234,602 13605 mRNA_5238: 13606 - KOG1199 Short-chain alcohol dehydrogenase/3-hydroxyacyl-CoA dehydrogenase, at 235,141 to 236,473 13606 mRNA_5239: 13607 - K10859 ALKBH2 alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, at 236,624 to 237,646 13607 mRNA_5240: 13608 - HMMPfam-Ubiquinol-cytochrome-c reductase complex subunit (QCR10)-PF09796, at 238,020 to 238,702 13608
Group Condition mRNA_5236 mRNA_5237 mRNA_5238 mRNA_5239 mRNA_5240
13604 13605 13606 13607 13608
ypd buoyancy 40 hrs Low Buoyancy -0.6 N.D. -0.2 -0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. -0.1 -0.3 -0.9
growth on oleic acid YNB Oleic Acid +0.1 N.D. -0.2 -0.4 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. -0.0 -0.0 -0.1
growth on oleic acid YNB Glucose -0.3 N.D. +0.1 -0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.0 -0.5 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. +0.6 -0.3 -0.6
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.2 -0.1 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.3 +0.4 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.1 N.D. +0.2 -0.6 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.3 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. +0.6 -0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. +0.6 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. +0.4 -0.1 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.1 +0.2 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.8 -0.2 -0.1
growth on ypd YPD +0.6 N.D. +0.1 -0.5 +0.3
auxotrophy Dropout Complete -0.1 N.D. +0.3 -0.2 +0.6
growth on ricinoleic acid YNB Glucose +0.4 N.D. +0.5 +0.1 +0.1
auxotrophy YNB Glucose +0.3 N.D. +0.4 +0.2 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. +0.5 +0.3 +0.7
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