Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13607 and 13608 are separated by 373 nucleotides13608 and 13609 are separated by 284 nucleotides13609 and 13610 are separated by 1023 nucleotides13610 and 13611 are separated by 2693 nucleotides mRNA_5239: 13607 - K10859 ALKBH2 alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, at 236,624 to 237,646 13607 mRNA_5240: 13608 - HMMPfam-Ubiquinol-cytochrome-c reductase complex subunit (QCR10)-PF09796, at 238,020 to 238,702 13608 mRNA_5241: 13609 - K01099 INPP5B_F inositol polyphosphate 5-phosphatase INPP5B/F, at 238,987 to 242,229 13609 mRNA_5242: 13610 - K02932 RP-L5e, RPL5 large subunit ribosomal protein L5e, at 243,253 to 244,505 13610 mRNA_5243: 13611 - KOG1601 GATA-4/5/6 transcription factors, at 247,199 to 251,043 13611
Group Condition mRNA_5239 mRNA_5240 mRNA_5241 mRNA_5242 mRNA_5243
13607 13608 13609 13610 13611
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.6 -0.4 N.D. -0.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 +0.0 -0.4 N.D. -0.3
growth on oleic acid YNB Oleic Acid -0.4 -0.2 -0.1 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.3 -0.1 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.1 -0.1 N.D. -0.4
growth on oleic acid YNB Glucose -0.1 -0.2 -0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 -0.6 -0.0 N.D. +0.4
growth on ypd YPD -0.5 +0.3 -0.3 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.3 +0.1 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.3 +0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.3 +0.1 N.D. +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.6 +0.3 -0.2 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.2 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.9 +0.3 N.D. +0.7
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.1 -0.4 N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.3 +0.0 N.D. +0.4
growth on ricinoleic acid YNB Glucose +0.1 +0.1 -0.1 N.D. -0.1
auxotrophy YNB Glucose +0.2 +0.3 -0.1 N.D. +0.1
auxotrophy Dropout Complete -0.2 +0.6 -0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.3 -0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.7 -0.1 N.D. +0.0
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