Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13610 and 13611 are separated by 2693 nucleotides13611 and 13612 are separated by 921 nucleotides13612 and 13613 are separated by 577 nucleotides13613 and 13614 are separated by 222 nucleotides mRNA_5242: 13610 - K02932 RP-L5e, RPL5 large subunit ribosomal protein L5e, at 243,253 to 244,505 13610 mRNA_5243: 13611 - KOG1601 GATA-4/5/6 transcription factors, at 247,199 to 251,043 13611 mRNA_5244: 13612 - HMMPfam-Glycosyl Hydrolase Family 88-PF07470,SUPERFAMILY--SSF48208, at 251,965 to 253,364 13612 mRNA_5245: 13613 - KOG2887 Membrane protein involved in ER to Golgi transport, at 253,942 to 254,889 13613 mRNA_5246: 13614 - K00411 UQCRFS1, RIP1, petA ubiquinol-cytochrome c reductase iron-sulfur subunit, at 255,112 to 256,642 13614
Group Condition mRNA_5242 mRNA_5243 mRNA_5244 mRNA_5245 mRNA_5246
13610 13611 13612 13613 13614
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 -0.3 N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.7 -0.3 N.D. -1.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 -0.1 N.D. -0.4
growth on oleic acid YNB Glucose N.D. -0.4 -0.2 N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.1 N.D. -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 -0.9 N.D. -0.2
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 -0.1 N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 -0.3 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 -0.3 N.D. +0.5
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 -0.1 N.D. -0.4
growth on ypd YPD N.D. -0.0 -0.3 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.0 -0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.1 N.D. +0.3
auxotrophy YNB Glucose N.D. +0.1 +0.0 N.D. +0.1
auxotrophy Dropout Complete N.D. +0.1 -0.0 N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.8 -0.3 N.D. +1.5
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 -0.1 N.D. +0.7
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 -0.3 N.D. +0.1
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