Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13614 and 13615 are separated by 296 nucleotides13615 and 13616 are separated by 804 nucleotides13616 and 13617 are separated by 449 nucleotides13617 and 13618 are separated by 2732 nucleotides mRNA_5246: 13614 - K00411 UQCRFS1, RIP1, petA ubiquinol-cytochrome c reductase iron-sulfur subunit, at 255,112 to 256,642 13614 mRNA_5247: 13615 - HMMPfam-Basic region leucine zipper-PF07716,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 256,939 to 258,074 13615 mRNA_5248: 13616 - KOG2084 Predicted histone tail methylase containing SET domain, at 258,879 to 260,199 13616 mRNA_5249: 13617 - K01535 PMA1, PMA2 H+-transporting ATPase, at 260,649 to 264,628 13617 mRNA_5250: 13618 - SUPERFAMILY--SSF101898, at 267,361 to 271,776 13618
Group Condition mRNA_5246 mRNA_5247 mRNA_5248 mRNA_5249 mRNA_5250
13614 13615 13616 13617 13618
facs on lipid accumulation Sorted High BODIPY Gate -1.3 +0.2 -0.3 N.D. -0.1
auxotrophy YNB Glucose +0.1 -0.5 -0.3 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.4 -0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.5 -0.1 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.1 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.2 -0.1 N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -0.2 +0.1 N.D. +0.2
growth on ricinoleic acid YNB Glucose -0.6 -0.0 +0.2 N.D. +0.0
growth on oleic acid YNB Glucose -0.2 -0.0 +0.0 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.0 +0.3 N.D. -0.0
auxotrophy Dropout Complete +0.3 -0.4 -0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.2 -0.0 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 -0.4 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.5 -0.3 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 +0.0 +0.2 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.5 -0.0 -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.2 -0.1 N.D. +0.1
growth on ypd YPD +0.2 +0.3 -0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.1 -0.0 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.7 +0.3 -0.5 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +1.5 +0.0 +0.2 N.D. +0.2
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