Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13617 and 13618 are separated by 2732 nucleotides13618 and 13619 are separated by 197 nucleotides13619 and 13620 are separated by 685 nucleotides13620 and 13621 are separated by 380 nucleotides mRNA_5249: 13617 - K01535 PMA1, PMA2 H+-transporting ATPase, at 260,649 to 264,628 13617 mRNA_5250: 13618 - SUPERFAMILY--SSF101898, at 267,361 to 271,776 13618 mRNA_5251: 13619 - K12867 SYF1, XAB2 pre-mRNA-splicing factor SYF1, at 271,974 to 275,743 13619 mRNA_5252: 13620 - BLAST LPS-assembly protein LptD [Rhizoctonia solani], at 276,429 to 278,610 13620 mRNA_5253: 13621 - Hypothetical Protein, at 278,991 to 279,822 13621
Group Condition mRNA_5249 mRNA_5250 mRNA_5251 mRNA_5252 mRNA_5253
13617 13618 13619 13620 13621
growth on ypd YPD N.D. +0.0 -1.3 -0.2 -0.3
auxotrophy YNB Glucose N.D. +0.1 -1.5 -0.0 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -1.1 -0.5 -0.3
auxotrophy Dropout Complete N.D. +0.1 -1.2 -0.2 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 -1.2 -0.1 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 -1.3 +0.0 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.8 -0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.8 +0.1 -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.6 +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 -0.9 -0.0 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 -1.0 +0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 +0.1 -0.5 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -0.3 -0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.7 +0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. +0.0 -0.5 +0.1 -0.1
growth on oleic acid YNB Glucose N.D. -0.1 +0.2 -0.2 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 +0.5 -0.4 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 +0.7 -0.3 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 -0.1 +0.0 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 +1.0 -0.6 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 +1.5 -0.6 -0.1
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