Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13622 and 13623 are separated by 289 nucleotides13623 and 13624 are separated by 1804 nucleotides13624 and 13625 are separated by 1986 nucleotides13625 and 13626 are separated by 199 nucleotides mRNA_5254: 13622 - HMMPfam-10 TM Acyl Transferase domain found in Cas1p-PF07779,HMMPfam-GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p-PF13839, at 280,539 to 283,748 13622 mRNA_5255: 13623 - K12604 CNOT1, NOT1 CCR4-NOT transcription complex subunit 1, at 284,038 to 290,339 13623 mRNA_5256: 13624 - SUPERFAMILY--SSF53448, at 292,144 to 293,863 13624 mRNA_5257: 13625 - K11887 PAAF1, RPN14 proteasomal ATPase-associated factor 1, at 295,850 to 297,525 13625 mRNA_5258: 13626 - KOG0538 Glycolate oxidase, at 297,725 to 299,742 13626
Group Condition mRNA_5254 mRNA_5255 mRNA_5256 mRNA_5257 mRNA_5258
13622 13623 13624 13625 13626
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. -0.5 -0.5 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. -0.3 +0.3 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. -0.7 +0.2 -0.4
auxotrophy Dropout Complete +0.0 N.D. +0.0 -0.1 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 N.D. +0.7 +0.4 -0.8
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.1 -0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. +0.3 -0.2 -0.4
growth on oleic acid YNB Oleic Acid +0.4 N.D. +0.3 -0.3 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.1 -0.1 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.8 -0.5 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.2 -0.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 N.D. +0.4 -0.6 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 N.D. +1.2 -0.9 -0.3
growth on oleic acid YNB Glucose +0.5 N.D. +0.5 +0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.3 +0.1 +0.4
auxotrophy YNB Glucose +0.3 N.D. +0.7 +0.2 +0.1
growth on ypd YPD +0.7 N.D. +0.5 +0.5 -0.4
growth on ricinoleic acid YNB Glucose +0.2 N.D. +1.0 +0.4 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 N.D. +1.2 -0.3 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 N.D. +1.5 +0.2 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. +1.7 +0.3 +0.2
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