Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13637 and 13638 are separated by 1344 nucleotides13638 and 13639 are separated by 534 nucleotides13639 and 13640 are separated by 216 nucleotides13640 and 13641 are separated by 438 nucleotides mRNA_5269: 13637 - K03320 amt, AMT, MEP ammonium transporter, Amt family, at 322,459 to 324,944 13637 mRNA_5270: 13638 - Hypothetical Protein, at 326,289 to 326,690 13638 mRNA_5271: 13639 - K11841 USP10, UBP3 ubiquitin carboxyl-terminal hydrolase 10, at 327,225 to 330,329 13639 mRNA_5272: 13640 - K02731 PSMA7 20S proteasome subunit alpha 4, at 330,546 to 332,091 13640 mRNA_5273: 13641 - K00004 BDH, butB (R,R)-butanediol dehydrogenase / meso-butanediol dehydrogenase / diacetyl reductase, at 332,530 to 334,206 13641
Group Condition mRNA_5269 mRNA_5270 mRNA_5271 mRNA_5272 mRNA_5273
13637 13638 13639 13640 13641
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 -0.5 -0.6 N.D. -0.4
growth on oleic acid YNB Glucose -0.3 -0.5 -1.1 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.5 -0.9 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.6 -0.5 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.1 -0.6 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.2 -1.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.1 -0.8 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.1 -0.8 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 -0.6 N.D. -0.1
auxotrophy YNB Glucose -0.2 +0.1 -0.5 N.D. -0.3
auxotrophy Dropout Complete +0.2 -0.2 -0.8 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.2 -0.2 -0.3 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.0 -0.2 N.D. -0.2
growth on oleic acid YNB Oleic Acid +0.3 -0.4 -0.6 N.D. +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.0 -0.6 N.D. +0.1
growth on ypd YPD +0.1 -0.4 +0.3 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 -0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.1 +0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.2 -0.0 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.2 -0.0 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.2 +0.2 N.D. +0.3
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