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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
13639 and 13640 are separated by 216 nucleotides
13640 and 13641 are separated by 438 nucleotides
13641 and 13642 are separated by 346 nucleotides
13642 and 13643 are separated by 334 nucleotides
mRNA_5271: 13639 - K11841 USP10, UBP3 ubiquitin carboxyl-terminal hydrolase 10, at 327,225 to 330,329
13639
mRNA_5272: 13640 - K02731 PSMA7 20S proteasome subunit alpha 4, at 330,546 to 332,091
13640
mRNA_5273: 13641 - K00004 BDH, butB (R,R)-butanediol dehydrogenase / meso-butanediol dehydrogenase / diacetyl reductase, at 332,530 to 334,206
13641
mRNA_5274: 13642 - KOG0032 Ca2+/calmodulin-dependent protein kinase, EF-Hand protein superfamily, at 334,553 to 337,184
13642
mRNA_5275: 13643 - K06688 UBE2C, UBC11 ubiquitin-conjugating enzyme E2 C, at 337,519 to 338,338
13643
Group
Condition
mRNA
_5271
mRNA
_5272
mRNA
_5273
mRNA
_5274
mRNA
_5275
13639
13640
13641
13642
13643
growth on oleic acid
YNB Glucose
-1.1
N.D.
+0.2
-1.2
-0.4
auxotrophy
YNB Glucose 75mM Arginine
-0.8
N.D.
-0.2
-1.3
-0.0
growth on oleic acid
YNB Oleic Acid
-0.6
N.D.
+0.0
-1.0
-0.7
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.6
N.D.
-0.1
-0.3
-1.0
auxotrophy
Dropout Complete
-0.8
N.D.
-0.1
-1.0
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.6
N.D.
+0.1
-1.5
+0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.8
N.D.
+0.0
-1.0
+0.3
facs on lipid accumulation
Sorted High BODIPY Gate
-1.1
N.D.
-0.0
-0.4
+0.0
auxotrophy
YNB Glucose
-0.5
N.D.
-0.3
-0.7
+0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.5
N.D.
+0.0
-0.3
-0.6
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
N.D.
-0.2
-0.2
-0.6
growth on ricinoleic acid
YNB Glucose
-0.3
N.D.
-0.2
-1.2
+0.6
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.0
N.D.
+0.0
-0.7
-0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.9
N.D.
+0.1
-1.0
+0.9
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
N.D.
-0.2
-0.3
-0.4
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.6
N.D.
-0.0
-0.3
+0.4
ypd buoyancy
40 hrs Low Buoyancy
-0.3
N.D.
+0.2
+0.0
-0.1
growth on ypd
YPD
+0.3
N.D.
-0.3
-0.3
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.6
N.D.
-0.4
+1.5
-0.5
ypd buoyancy
40 hrs Median Buoyancy
-0.0
N.D.
+0.3
+0.1
-0.1
ypd buoyancy
40 hrs High Buoyancy
+0.2
N.D.
+0.3
+0.3
-0.0
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