Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13640 and 13641 are separated by 438 nucleotides13641 and 13642 are separated by 346 nucleotides13642 and 13643 are separated by 334 nucleotides13643 and 13644 are separated by 920 nucleotides mRNA_5272: 13640 - K02731 PSMA7 20S proteasome subunit alpha 4, at 330,546 to 332,091 13640 mRNA_5273: 13641 - K00004 BDH, butB (R,R)-butanediol dehydrogenase / meso-butanediol dehydrogenase / diacetyl reductase, at 332,530 to 334,206 13641 mRNA_5274: 13642 - KOG0032 Ca2+/calmodulin-dependent protein kinase, EF-Hand protein superfamily, at 334,553 to 337,184 13642 mRNA_5275: 13643 - K06688 UBE2C, UBC11 ubiquitin-conjugating enzyme E2 C, at 337,519 to 338,338 13643 mRNA_5276: 13644 - Hypothetical Protein, at 339,259 to 340,241 13644
Group Condition mRNA_5272 mRNA_5273 mRNA_5274 mRNA_5275 mRNA_5276
13640 13641 13642 13643 13644
growth on oleic acid YNB Glucose N.D. +0.2 -1.2 -0.4 -0.3
growth on oleic acid YNB Oleic Acid N.D. +0.0 -1.0 -0.7 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -1.3 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.3 -1.0 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -1.5 +0.3 -0.2
auxotrophy YNB Glucose N.D. -0.3 -0.7 +0.0 -0.2
auxotrophy Dropout Complete N.D. -0.1 -1.0 -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -1.0 +0.3 -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.2 -1.2 +0.6 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.2 -0.6 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.3 -0.4 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -0.3 -0.6 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.0 -0.7 -0.3 +0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 -0.4 +0.0 -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 +0.0 -0.1 -0.1
growth on ypd YPD N.D. -0.3 -0.3 +0.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 -0.3 +0.4 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 +0.1 -0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -1.0 +0.9 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 +0.3 -0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 +1.5 -0.5 +0.4
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