Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13648 and 13649 are separated by 771 nucleotides13649 and 13650 are separated by 289 nucleotides13650 and 13651 are separated by 152 nucleotides13651 and 13652 are separated by 1477 nucleotides mRNA_5280: 13648 - KOG0085 G protein subunit Galphaq/Galphay, small G protein superfamily, at 348,551 to 349,893 13648 mRNA_5281: 13649 - KOG0085 G protein subunit Galphaq/Galphay, small G protein superfamily, at 350,665 to 352,490 13649 mRNA_5282: 13650 - Hypothetical Protein, at 352,780 to 353,603 13650 mRNA_5283: 13651 - Hypothetical Protein, at 353,756 to 354,946 13651 mRNA_5284: 13652 - Hypothetical Protein, at 356,424 to 357,463 13652
Group Condition mRNA_5280 mRNA_5281 mRNA_5282 mRNA_5283 mRNA_5284
13648 13649 13650 13651 13652
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.1 N.D. -0.4 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.1 N.D. +0.0 -0.5
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 N.D. -0.2 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 N.D. -0.1 -0.8
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.0 N.D. +0.1 -0.5
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.0 N.D. -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 N.D. -0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.1 N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.3 N.D. -0.2 -0.3
growth on oleic acid YNB Oleic Acid +0.2 -0.2 N.D. +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 -0.2 N.D. -0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.7 N.D. -1.5 +1.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 N.D. -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.0 N.D. -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 N.D. -0.3 +0.3
auxotrophy Dropout Complete -0.1 +0.1 N.D. +0.0 +0.3
growth on ricinoleic acid YNB Glucose +0.3 +0.0 N.D. -0.0 +0.1
auxotrophy YNB Glucose -0.3 +0.1 N.D. +0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 N.D. +0.5 +0.2
growth on oleic acid YNB Glucose +0.3 -0.0 N.D. +0.3 +0.5
growth on ypd YPD +1.1 +0.0 N.D. +0.7 +0.5
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