Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13657 and 13658 are separated by 421 nucleotides13658 and 13659 are separated by 397 nucleotides13659 and 13660 are separated by 624 nucleotides13660 and 13661 are separated by 493 nucleotides mRNA_5289: 13657 - K00344 qor, CRYZ NADPH2-quinone reductase, at 363,937 to 365,504 13657 mRNA_5290: 13658 - HMMPfam-ARID/BRIGHT DNA binding domain-PF01388,ProSiteProfiles-ARID domain profile.-PS51011,SMART-BRIGHT, ARID (A/T-rich interaction domain) domain-SM00501,SMART-ARID/BRIGHT DNA binding domain-SM01014,SUPERFAMILY--SSF46774, at 365,926 to 369,880 13658 mRNA_5291: 13659 - K00799 GST, gst glutathione S-transferase, at 370,278 to 371,436 13659 mRNA_5292: 13660 - Hypothetical Protein, at 372,061 to 373,005 13660 mRNA_5293: 13661 - Hypothetical Protein, at 373,499 to 378,247 13661
Group Condition mRNA_5289 mRNA_5290 mRNA_5291 mRNA_5292 mRNA_5293
13657 13658 13659 13660 13661
growth on ypd YPD +0.2 +0.1 -0.2 -0.3 +0.0
growth on ricinoleic acid YNB Glucose -0.2 -0.1 -0.1 +0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.1 +0.0 +0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 -0.2 -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.0 +0.0 +0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.0 -0.3 +0.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.2 +0.1 +0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.0 +0.3 +0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.1 -0.3 +0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.0 -0.1 -0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.2 -0.2 +0.0 +0.2
auxotrophy Dropout Complete +0.4 -0.1 +0.1 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 +0.3 +0.3 +0.0 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.4 -0.3 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.1 +0.6 +0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.2 +0.3 +0.1 +0.0
growth on oleic acid YNB Glucose +0.1 -0.1 +0.1 +0.2 +0.1
auxotrophy YNB Glucose +0.4 -0.0 +0.2 -0.2 +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 +0.2 +0.2 -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.7 -0.0 +0.0 -0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.1 +0.4 -0.0 +0.0
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