Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13658 and 13659 are separated by 397 nucleotides13659 and 13660 are separated by 624 nucleotides13660 and 13661 are separated by 493 nucleotides13661 and 13662 are separated by 376 nucleotides mRNA_5290: 13658 - HMMPfam-ARID/BRIGHT DNA binding domain-PF01388,ProSiteProfiles-ARID domain profile.-PS51011,SMART-BRIGHT, ARID (A/T-rich interaction domain) domain-SM00501,SMART-ARID/BRIGHT DNA binding domain-SM01014,SUPERFAMILY--SSF46774, at 365,926 to 369,880 13658 mRNA_5291: 13659 - K00799 GST, gst glutathione S-transferase, at 370,278 to 371,436 13659 mRNA_5292: 13660 - Hypothetical Protein, at 372,061 to 373,005 13660 mRNA_5293: 13661 - Hypothetical Protein, at 373,499 to 378,247 13661 mRNA_5294: 13662 - K02830 HRAD1, RAD17 cell cycle checkpoint protein, at 378,624 to 380,568 13662
Group Condition mRNA_5290 mRNA_5291 mRNA_5292 mRNA_5293 mRNA_5294
13658 13659 13660 13661 13662
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.3 -0.2 +0.1 -4.3
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.1 -0.3 +0.2 -1.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.6 +0.2 -0.0 -2.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.0 -0.0 -0.0 -1.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.1 +0.3 -0.0 -1.1
growth on ricinoleic acid YNB Glucose -0.1 -0.1 +0.2 -0.1 -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.0 +0.2 -0.2 -1.0
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.2 -0.2 +0.2 -1.2
growth on ypd YPD +0.1 -0.2 -0.3 +0.0 -0.4
growth on oleic acid YNB Oleic Acid -0.1 +0.0 +0.1 -0.2 -0.6
auxotrophy Dropout Complete -0.1 +0.1 -0.1 -0.0 -0.5
auxotrophy YNB Glucose -0.0 +0.2 -0.2 +0.1 -0.8
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.4 -0.0 +0.0 -0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.3 +0.0 -0.1 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.3 +0.0 -0.1 -0.8
growth on oleic acid YNB Glucose -0.1 +0.1 +0.2 +0.1 -0.6
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 -0.1 -0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.2 +0.0 +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.3 +0.1 +0.0 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.3 +0.1 +0.2 +0.4
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.3 +0.1 +0.0 +0.6
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