Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13665 and 13666 are separated by 2322 nucleotides13666 and 13667 are separated by 335 nucleotides13667 and 13668 are separated by 550 nucleotides13668 and 13669 are separated by 190 nucleotides mRNA_5297: 13665 - Hypothetical Protein, at 383,150 to 384,089 13665 mRNA_5298: 13666 - KOG0758 Mitochondrial carnitine-acylcarnitine carrier protein, at 386,412 to 388,025 13666 mRNA_5299: 13667 - BLAST RNA polymerase sigma factor, sigma-70 family [Rho..., at 388,361 to 391,277 13667 mRNA_5300: 13668 - K03023 RPC3, POLR3C DNA-directed RNA polymerase III subunit RPC3, at 391,828 to 394,452 13668 mRNA_5301: 13669 - K13950 pabAB para-aminobenzoate synthetase, at 394,643 to 397,418 13669
Group Condition mRNA_5297 mRNA_5298 mRNA_5299 mRNA_5300 mRNA_5301
13665 13666 13667 13668 13669
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.3 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.5 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. -0.3 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 N.D. -0.2 N.D. -0.1
auxotrophy YNB Glucose -0.0 N.D. -0.3 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine -0.0 N.D. -0.1 N.D. -0.1
growth on ypd YPD +0.1 N.D. -0.1 N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. -0.0 N.D. -0.0
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.2 N.D. +0.0
auxotrophy Dropout Complete +0.1 N.D. -0.1 N.D. +0.0
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. -0.2 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 N.D. -0.0 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy +0.3 N.D. +0.0 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. +0.2 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.3 N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.1 N.D. +0.4 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.2 N.D. +0.2
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