Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13684 and 13685 are separated by 242 nucleotides13685 and 13686 are separated by 82 nucleotides13686 and 13687 are separated by 712 nucleotides13687 and 13688 are separated by 345 nucleotides mRNA_5316: 13684 - Hypothetical Protein, at 440,018 to 441,628 13684 mRNA_5317: 13685 - K17498 SPN1, IWS1 transcription factor SPN1, at 441,871 to 444,778 13685 mRNA_5318: 13686 - KOG4315 G-patch nucleic acid binding protein, at 444,861 to 446,150 13686 mRNA_5319: 13687 - HMMPfam-Plasma-membrane choline transporter-PF04515, at 446,863 to 449,105 13687 mRNA_5320: 13688 - K03038 PSMD7, RPN8 26S proteasome regulatory subunit N8, at 449,451 to 450,740 13688
Group Condition mRNA_5316 mRNA_5317 mRNA_5318 mRNA_5319 mRNA_5320
13684 13685 13686 13687 13688
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.1 N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.6 N.D. -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.5 N.D. -0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.2 N.D. -0.6 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.1 N.D. -0.2 N.D.
auxotrophy YNB Glucose +0.0 -0.1 N.D. -0.3 N.D.
growth on oleic acid YNB Glucose -0.2 +0.0 N.D. -0.2 N.D.
growth on ypd YPD -0.3 -0.2 N.D. +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.4 N.D. -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.2 N.D. -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.0 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 N.D. -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.1 N.D. -0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.2 +0.1 N.D. -0.3 N.D.
auxotrophy Dropout Complete +0.3 -0.1 N.D. -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.0 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.3 -0.2 N.D. +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 +0.1 N.D. -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.2 N.D. +0.3 N.D.
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