Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13686 and 13687 are separated by 712 nucleotides13687 and 13688 are separated by 345 nucleotides13688 and 13689 are separated by 423 nucleotides13689 and 13690 are separated by 146 nucleotides mRNA_5318: 13686 - KOG4315 G-patch nucleic acid binding protein, at 444,861 to 446,150 13686 mRNA_5319: 13687 - HMMPfam-Plasma-membrane choline transporter-PF04515, at 446,863 to 449,105 13687 mRNA_5320: 13688 - K03038 PSMD7, RPN8 26S proteasome regulatory subunit N8, at 449,451 to 450,740 13688 mRNA_5321: 13689 - K11108 RCL1 RNA 3'-terminal phosphate cyclase-like protein, at 451,164 to 452,723 13689 mRNA_5322: 13690 - K01120 cpdP 3',5'-cyclic-nucleotide phosphodiesterase, at 452,870 to 455,692 13690
Group Condition mRNA_5318 mRNA_5319 mRNA_5320 mRNA_5321 mRNA_5322
13686 13687 13688 13689 13690
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. N.D. -0.9
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.6 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 N.D. N.D. -0.6
growth on oleic acid YNB Oleic Acid N.D. -0.3 N.D. N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 N.D. N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.5 N.D. N.D. +0.3
growth on oleic acid YNB Glucose N.D. -0.2 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. N.D. +0.1
auxotrophy YNB Glucose N.D. -0.3 N.D. N.D. +0.2
growth on ypd YPD N.D. +0.3 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 N.D. N.D. +0.4
auxotrophy Dropout Complete N.D. -0.0 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 N.D. N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 N.D. N.D. -0.1
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