Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13689 and 13690 are separated by 146 nucleotides13690 and 13691 are separated by 373 nucleotides13691 and 13692 are separated by 140 nucleotides13692 and 13693 are separated by 483 nucleotides mRNA_5321: 13689 - K11108 RCL1 RNA 3'-terminal phosphate cyclase-like protein, at 451,164 to 452,723 13689 mRNA_5322: 13690 - K01120 cpdP 3',5'-cyclic-nucleotide phosphodiesterase, at 452,870 to 455,692 13690 mRNA_5323: 13691 - K10842 MNAT1 CDK-activating kinase assembly factor MAT1, at 456,066 to 457,717 13691 mRNA_5324: 13692 - KOG2852 Possible oxidoreductase, at 457,858 to 459,287 13692 mRNA_5325: 13693 - HMMPfam-GDSL-like Lipase/Acylhydrolase-PF00657,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF52266, at 459,771 to 461,459 13693
Group Condition mRNA_5321 mRNA_5322 mRNA_5323 mRNA_5324 mRNA_5325
13689 13690 13691 13692 13693
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 N.D. -0.3 -0.6
growth on oleic acid YNB Glucose N.D. -0.1 N.D. -0.3 -0.5
growth on ricinoleic acid YNB Glucose N.D. -0.3 N.D. -0.4 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.9 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. -0.2 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 N.D. +0.2 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 N.D. -0.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. +0.3 -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.3 N.D. +0.0 -0.1
growth on ypd YPD N.D. -0.3 N.D. +0.3 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 N.D. -0.1 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 N.D. -0.5 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. -0.5 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 N.D. +0.0 +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. +0.2 +0.3
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 N.D. -0.1 +0.1
auxotrophy Dropout Complete N.D. +0.2 N.D. +0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.6 +0.4
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