Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13691 and 13692 are separated by 140 nucleotides13692 and 13693 are separated by 483 nucleotides13693 and 13694 are separated by 288 nucleotides13694 and 13695 are separated by 251 nucleotides mRNA_5323: 13691 - K10842 MNAT1 CDK-activating kinase assembly factor MAT1, at 456,066 to 457,717 13691 mRNA_5324: 13692 - KOG2852 Possible oxidoreductase, at 457,858 to 459,287 13692 mRNA_5325: 13693 - HMMPfam-GDSL-like Lipase/Acylhydrolase-PF00657,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257,SUPERFAMILY--SSF52266, at 459,771 to 461,459 13693 mRNA_5326: 13694 - K00020 mmsB, HIBADH 3-hydroxyisobutyrate dehydrogenase, at 461,748 to 463,259 13694 mRNA_5327: 13695 - Hypothetical Protein, at 463,511 to 464,845 13695
Group Condition mRNA_5323 mRNA_5324 mRNA_5325 mRNA_5326 mRNA_5327
13691 13692 13693 13694 13695
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 -0.1 -0.2 -2.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.3 +0.0 -0.1 -0.8
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 +0.4 -0.4 -0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 +0.3 -0.3 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 -0.6 -0.4 +0.7
growth on oleic acid YNB Glucose N.D. -0.3 -0.5 -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.3 -0.4 +0.0 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.2 -0.2 -0.4 +0.4
auxotrophy YNB Glucose N.D. -0.1 +0.3 -0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.0 -0.2 -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.4 -0.1 -0.2 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.3 -0.1 +0.3
growth on oleic acid YNB Oleic Acid N.D. +0.0 -0.1 -0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.0 +0.1 -0.2 -0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 +0.3 -0.1 -0.5
auxotrophy Dropout Complete N.D. +0.0 +0.2 -0.1 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 +0.1 +0.2 -0.3
growth on ypd YPD N.D. +0.3 -0.2 -0.0 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.1 -0.1 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 +0.0 -0.2 +0.7
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.6 +0.4 -0.2 -0.2
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