Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13701 and 13702 are separated by 246 nucleotides13702 and 13703 are separated by 299 nucleotides13703 and 13704 are separated by 279 nucleotides13704 and 13705 are separated by 215 nucleotides mRNA_5333: 13701 - ProSiteProfiles-Ribonuclease III family domain profile.-PS50142,SUPERFAMILY--SSF69065, at 478,191 to 479,250 13701 mRNA_5334: 13702 - K14563 NOP1, FBL rRNA 2'-O-methyltransferase fibrillarin, at 479,497 to 481,330 13702 mRNA_5335: 13703 - HMMPfam-Domain of unknown function (DUF4202)-PF13875, at 481,630 to 482,657 13703 mRNA_5336: 13704 - K14777 DDX47, RRP3 ATP-dependent RNA helicase DDX47/RRP3, at 482,937 to 484,688 13704 mRNA_5337: 13705 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 484,904 to 487,998 13705
Group Condition mRNA_5333 mRNA_5334 mRNA_5335 mRNA_5336 mRNA_5337
13701 13702 13703 13704 13705
ypd buoyancy 40 hrs Median Buoyancy -0.1 -1.2 -0.5 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.3 -1.1 -0.5 N.D. +0.2
growth on oleic acid YNB Oleic Acid -0.2 -1.2 +0.5 N.D. -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.4 -0.0 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.5 +0.1 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.5 +0.2 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 +0.6 +0.2 N.D. -0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.4 +0.5 N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.2 -0.2 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.1 +0.2 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.3 +0.2 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.3 +0.6 N.D. -0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.4 +0.4 N.D. -0.3
growth on oleic acid YNB Glucose -0.1 +0.2 +0.5 N.D. +0.1
auxotrophy Dropout Complete -0.1 -0.2 +1.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +1.7 -0.6 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +1.7 -0.9 N.D. -0.1
growth on ypd YPD -0.1 +1.5 -0.1 N.D. -0.3
auxotrophy YNB Glucose +0.3 -0.0 +1.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +2.0 -0.5 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +2.1 -0.1 N.D. -0.2
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