Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13702 and 13703 are separated by 299 nucleotides13703 and 13704 are separated by 279 nucleotides13704 and 13705 are separated by 215 nucleotides13705 and 13706 are separated by 483 nucleotides mRNA_5334: 13702 - K14563 NOP1, FBL rRNA 2'-O-methyltransferase fibrillarin, at 479,497 to 481,330 13702 mRNA_5335: 13703 - HMMPfam-Domain of unknown function (DUF4202)-PF13875, at 481,630 to 482,657 13703 mRNA_5336: 13704 - K14777 DDX47, RRP3 ATP-dependent RNA helicase DDX47/RRP3, at 482,937 to 484,688 13704 mRNA_5337: 13705 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 484,904 to 487,998 13705 mRNA_5338: 13706 - K00891 E2.7.1.71, aroK, aroL shikimate kinase, at 488,482 to 491,424 13706
Group Condition mRNA_5334 mRNA_5335 mRNA_5336 mRNA_5337 mRNA_5338
13702 13703 13704 13705 13706
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.0 N.D. -0.2 -1.7
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.2 N.D. -0.1 -1.6
ypd buoyancy 40 hrs Median Buoyancy -1.2 -0.5 N.D. -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy -1.1 -0.5 N.D. +0.2 +0.2
growth on oleic acid YNB Oleic Acid -1.2 +0.5 N.D. -0.0 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 +0.2 N.D. -0.6 -1.1
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.1 N.D. -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.6 N.D. -0.2 -0.6
growth on ricinoleic acid YNB Glucose +0.2 -0.2 N.D. -0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.4 N.D. -0.3 -0.8
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 N.D. -0.0 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 N.D. +0.3 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.5 N.D. -0.1 +0.3
growth on oleic acid YNB Glucose +0.2 +0.5 N.D. +0.1 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +1.7 -0.9 N.D. -0.1 -0.1
auxotrophy YNB Glucose -0.0 +1.1 N.D. -0.1 -0.4
auxotrophy Dropout Complete -0.2 +1.0 N.D. +0.1 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +1.7 -0.6 N.D. -0.2 -0.1
growth on ypd YPD +1.5 -0.1 N.D. -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +2.1 -0.1 N.D. -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +2.0 -0.5 N.D. -0.0 +0.1
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