Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13703 and 13704 are separated by 279 nucleotides13704 and 13705 are separated by 215 nucleotides13705 and 13706 are separated by 483 nucleotides13706 and 13707 are separated by 153 nucleotides mRNA_5335: 13703 - HMMPfam-Domain of unknown function (DUF4202)-PF13875, at 481,630 to 482,657 13703 mRNA_5336: 13704 - K14777 DDX47, RRP3 ATP-dependent RNA helicase DDX47/RRP3, at 482,937 to 484,688 13704 mRNA_5337: 13705 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 484,904 to 487,998 13705 mRNA_5338: 13706 - K00891 E2.7.1.71, aroK, aroL shikimate kinase, at 488,482 to 491,424 13706 mRNA_5339: 13707 - KOG4771 Nucleolar protein (NOP16) involved in 60S ribosomal subunit biogenesis, at 491,578 to 492,823 13707
Group Condition mRNA_5335 mRNA_5336 mRNA_5337 mRNA_5338 mRNA_5339
13703 13704 13705 13706 13707
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 N.D. -0.2 -1.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.1 -1.6 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -0.6 -1.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.9 N.D. -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 N.D. -0.2 -0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.2 N.D. -0.1 -0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.4 N.D. -0.3 -0.8 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. -0.2 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.5 N.D. -0.1 +0.1 N.D.
growth on ypd YPD -0.1 N.D. -0.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. -0.0 +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.6 N.D. -0.2 -0.6 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.5 N.D. +0.2 +0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.1 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. -0.0 -0.2 N.D.
growth on oleic acid YNB Glucose +0.5 N.D. +0.1 -0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.5 N.D. -0.0 -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. +0.3 -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.5 N.D. -0.1 +0.3 N.D.
auxotrophy YNB Glucose +1.1 N.D. -0.1 -0.4 N.D.
auxotrophy Dropout Complete +1.0 N.D. +0.1 -0.1 N.D.
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