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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
13703 and 13704 are separated by 279 nucleotides
13704 and 13705 are separated by 215 nucleotides
13705 and 13706 are separated by 483 nucleotides
13706 and 13707 are separated by 153 nucleotides
mRNA_5335: 13703 - HMMPfam-Domain of unknown function (DUF4202)-PF13875, at 481,630 to 482,657
13703
mRNA_5336: 13704 - K14777 DDX47, RRP3 ATP-dependent RNA helicase DDX47/RRP3, at 482,937 to 484,688
13704
mRNA_5337: 13705 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 484,904 to 487,998
13705
mRNA_5338: 13706 - K00891 E2.7.1.71, aroK, aroL shikimate kinase, at 488,482 to 491,424
13706
mRNA_5339: 13707 - KOG4771 Nucleolar protein (NOP16) involved in 60S ribosomal subunit biogenesis, at 491,578 to 492,823
13707
Group
Condition
mRNA
_5335
mRNA
_5336
mRNA
_5337
mRNA
_5338
mRNA
_5339
13703
13704
13705
13706
13707
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.0
N.D.
-0.2
-1.7
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.2
N.D.
-0.1
-1.6
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
N.D.
-0.6
-1.1
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.9
N.D.
-0.1
-0.1
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.6
N.D.
-0.2
-0.1
N.D.
growth on ricinoleic acid
YNB Glucose
-0.2
N.D.
-0.1
-0.5
N.D.
facs on lipid accumulation
Sorted No BODIPY Gate
+0.4
N.D.
-0.3
-0.8
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.1
N.D.
-0.2
-0.2
N.D.
ypd buoyancy
40 hrs Median Buoyancy
-0.5
N.D.
-0.1
+0.1
N.D.
growth on ypd
YPD
-0.1
N.D.
-0.3
-0.0
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.5
N.D.
-0.0
+0.1
N.D.
auxotrophy
YNB Glucose 75mM Methionine
+0.6
N.D.
-0.2
-0.6
N.D.
ypd buoyancy
40 hrs High Buoyancy
-0.5
N.D.
+0.2
+0.2
N.D.
ypd buoyancy
40 hrs Low Buoyancy
+0.1
N.D.
-0.1
-0.1
N.D.
auxotrophy
YNB Glucose 75mM Arginine
+0.2
N.D.
-0.0
-0.2
N.D.
growth on oleic acid
YNB Glucose
+0.5
N.D.
+0.1
-0.4
N.D.
growth on oleic acid
YNB Oleic Acid
+0.5
N.D.
-0.0
-0.3
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
N.D.
+0.3
-0.1
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
+0.5
N.D.
-0.1
+0.3
N.D.
auxotrophy
YNB Glucose
+1.1
N.D.
-0.1
-0.4
N.D.
auxotrophy
Dropout Complete
+1.0
N.D.
+0.1
-0.1
N.D.
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