Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13704 and 13705 are separated by 215 nucleotides13705 and 13706 are separated by 483 nucleotides13706 and 13707 are separated by 153 nucleotides13707 and 13708 are separated by 178 nucleotides mRNA_5336: 13704 - K14777 DDX47, RRP3 ATP-dependent RNA helicase DDX47/RRP3, at 482,937 to 484,688 13704 mRNA_5337: 13705 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 484,904 to 487,998 13705 mRNA_5338: 13706 - K00891 E2.7.1.71, aroK, aroL shikimate kinase, at 488,482 to 491,424 13706 mRNA_5339: 13707 - KOG4771 Nucleolar protein (NOP16) involved in 60S ribosomal subunit biogenesis, at 491,578 to 492,823 13707 mRNA_5340: 13708 - K01092 E3.1.3.25, IMPA, suhB myo-inositol-1(or 4)-monophosphatase, at 493,002 to 494,592 13708
Group Condition mRNA_5336 mRNA_5337 mRNA_5338 mRNA_5339 mRNA_5340
13704 13705 13706 13707 13708
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.6 -1.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 -1.7 N.D. +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 -1.6 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.8 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.2 -0.6 N.D. -0.2
growth on ypd YPD N.D. -0.3 -0.0 N.D. -0.5
auxotrophy YNB Glucose N.D. -0.1 -0.4 N.D. -0.1
growth on oleic acid YNB Oleic Acid N.D. -0.0 -0.3 N.D. -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.1 -0.5 N.D. +0.2
growth on oleic acid YNB Glucose N.D. +0.1 -0.4 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 -0.2 N.D. -0.0
auxotrophy Dropout Complete N.D. +0.1 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.2 -0.2 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -0.1 N.D. +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 -0.1 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.1 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.1 +0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 +0.1 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 +0.2 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 -0.1 N.D. +0.2
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