Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13705 and 13706 are separated by 483 nucleotides13706 and 13707 are separated by 153 nucleotides13707 and 13708 are separated by 178 nucleotides13708 and 13709 are separated by 646 nucleotides mRNA_5337: 13705 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 484,904 to 487,998 13705 mRNA_5338: 13706 - K00891 E2.7.1.71, aroK, aroL shikimate kinase, at 488,482 to 491,424 13706 mRNA_5339: 13707 - KOG4771 Nucleolar protein (NOP16) involved in 60S ribosomal subunit biogenesis, at 491,578 to 492,823 13707 mRNA_5340: 13708 - K01092 E3.1.3.25, IMPA, suhB myo-inositol-1(or 4)-monophosphatase, at 493,002 to 494,592 13708 mRNA_5341: 13709 - KOG0692 Pentafunctional AROM protein, at 495,239 to 496,655 13709
Group Condition mRNA_5337 mRNA_5338 mRNA_5339 mRNA_5340 mRNA_5341
13705 13706 13707 13708 13709
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -1.1 N.D. -0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -1.7 N.D. +0.2 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -1.6 N.D. +0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.6 N.D. -0.2 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.8 N.D. +0.1 -0.0
growth on ypd YPD -0.3 -0.0 N.D. -0.5 -0.0
auxotrophy YNB Glucose -0.1 -0.4 N.D. -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.2 N.D. +0.3 -0.4
growth on ricinoleic acid YNB Glucose -0.1 -0.5 N.D. +0.2 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.2 N.D. -0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.3 N.D. +0.2 -0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.1 N.D. +0.3 -0.4
growth on oleic acid YNB Glucose +0.1 -0.4 N.D. +0.0 +0.0
growth on oleic acid YNB Oleic Acid -0.0 -0.3 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.1 N.D. +0.3 -0.1
auxotrophy Dropout Complete +0.1 -0.1 N.D. -0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.1 N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.0 +0.1 N.D. +0.3 -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.1 N.D. +0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.2 N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.2 +0.1
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