Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13706 and 13707 are separated by 153 nucleotides13707 and 13708 are separated by 178 nucleotides13708 and 13709 are separated by 646 nucleotides13709 and 13710 are separated by 237 nucleotides mRNA_5338: 13706 - K00891 E2.7.1.71, aroK, aroL shikimate kinase, at 488,482 to 491,424 13706 mRNA_5339: 13707 - KOG4771 Nucleolar protein (NOP16) involved in 60S ribosomal subunit biogenesis, at 491,578 to 492,823 13707 mRNA_5340: 13708 - K01092 E3.1.3.25, IMPA, suhB myo-inositol-1(or 4)-monophosphatase, at 493,002 to 494,592 13708 mRNA_5341: 13709 - KOG0692 Pentafunctional AROM protein, at 495,239 to 496,655 13709 mRNA_5342: 13710 - KOG0692 Pentafunctional AROM protein, at 496,893 to 498,783 13710
Group Condition mRNA_5338 mRNA_5339 mRNA_5340 mRNA_5341 mRNA_5342
13706 13707 13708 13709 13710
growth on ricinoleic acid YNB Ricinoleic Acid -1.7 N.D. +0.2 -0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -1.6 N.D. +0.1 -0.0 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.6 N.D. -0.2 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.1 N.D. -0.2 +0.1 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +0.2 -0.7 -0.4
growth on oleic acid YNB Glucose -0.4 N.D. +0.0 +0.0 -0.2
growth on ypd YPD -0.0 N.D. -0.5 -0.0 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 N.D. +0.3 -0.4 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.0 -0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. +0.1 -0.0 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.3 -0.1 -0.4
auxotrophy YNB Glucose -0.4 N.D. -0.1 -0.1 +0.2
growth on ricinoleic acid YNB Glucose -0.5 N.D. +0.2 -0.1 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.3 -0.5 -0.3
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.0 +0.1 -0.1
auxotrophy Dropout Complete -0.1 N.D. -0.1 +0.0 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. +0.3 -0.4 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. +0.2 -0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.2 +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.1 -0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. +0.2 -0.2 +0.3
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