Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13716 and 13717 are separated by 133 nucleotides13717 and 13718 are separated by 444 nucleotides13718 and 13719 are separated by 751 nucleotides13719 and 13720 are separated by 2414 nucleotides mRNA_5348: 13716 - BLAST transmembrane protein, putative [Rhizoctonia solani A..., at 512,540 to 514,284 13716 mRNA_5349: 13717 - K17427 MRPL46 large subunit ribosomal protein L46, at 514,418 to 515,269 13717 mRNA_5350: 13718 - K20310 TRAPPC13 trafficking protein particle complex subunit 13, at 515,714 to 517,641 13718 mRNA_5351: 13719 - BLAST transmembrane protein, putative [Rhizoctonia solani A..., at 518,393 to 520,382 13719 mRNA_5352: 13720 - KOG1674 Cyclin, at 522,797 to 523,590 13720
Group Condition mRNA_5348 mRNA_5349 mRNA_5350 mRNA_5351 mRNA_5352
13716 13717 13718 13719 13720
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.1 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.1 -0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.2 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -0.3 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. +0.3 -0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 N.D. -0.2 +0.1 N.D.
growth on oleic acid YNB Glucose -0.1 N.D. +0.1 +0.1 N.D.
auxotrophy YNB Glucose +0.3 N.D. +0.0 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.2 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.2 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. -0.1 +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 N.D. -0.1 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.2 +0.1 N.D.
auxotrophy Dropout Complete +0.3 N.D. +0.4 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. +0.1 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.2 +0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.3 N.D. +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. +0.4 +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.6 N.D. +0.1 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.3 +0.2 N.D.
growth on ypd YPD +0.2 N.D. +0.3 +0.4 N.D.
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