Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13718 and 13719 are separated by 751 nucleotides13719 and 13720 are separated by 2414 nucleotides13720 and 13721 are separated by 754 nucleotides13721 and 13722 are separated by 1024 nucleotides mRNA_5350: 13718 - K20310 TRAPPC13 trafficking protein particle complex subunit 13, at 515,714 to 517,641 13718 mRNA_5351: 13719 - BLAST transmembrane protein, putative [Rhizoctonia solani A..., at 518,393 to 520,382 13719 mRNA_5352: 13720 - KOG1674 Cyclin, at 522,797 to 523,590 13720 mRNA_5353: 13721 - Hypothetical Protein, at 524,345 to 526,185 13721 mRNA_5354: 13722 - KOG0557 Dihydrolipoamide acetyltransferase, at 527,210 to 528,729 13722
Group Condition mRNA_5350 mRNA_5351 mRNA_5352 mRNA_5353 mRNA_5354
13718 13719 13720 13721 13722
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 N.D. -0.2 -1.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.1 N.D. -0.5 -1.1
growth on oleic acid YNB Glucose +0.1 +0.1 N.D. -0.0 -1.2
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.3 N.D. -0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.0 N.D. -0.4 -0.1
auxotrophy Dropout Complete +0.4 -0.3 N.D. -0.6 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.2 N.D. -0.3 -0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.0 N.D. -0.1 -0.7
auxotrophy YNB Glucose +0.0 -0.2 N.D. -0.4 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.2 N.D. +0.1 -0.9
growth on ricinoleic acid YNB Glucose +0.1 +0.2 N.D. -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 +0.2 N.D. -0.0 -0.3
growth on oleic acid YNB Oleic Acid -0.2 +0.3 N.D. -0.1 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 N.D. -0.3 +0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.2 N.D. -0.3 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 N.D. -0.3 +0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.4 N.D. +0.2 +0.6
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 N.D. +0.1 +1.2
growth on ypd YPD +0.3 +0.4 N.D. +0.7 +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.1 N.D. +0.4 +1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.3 N.D. -0.3 +2.4
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