Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13725 and 13726 are separated by 307 nucleotides13726 and 13727 are separated by 314 nucleotides13727 and 13728 are separated by 537 nucleotides13728 and 13729 are separated by 539 nucleotides mRNA_5357: 13725 - HMMPfam-Methyltransferase domain-PF12847,SUPERFAMILY--SSF53335, at 533,948 to 534,977 13725 mRNA_5358: 13726 - Hypothetical Protein, at 535,285 to 536,423 13726 mRNA_5359: 13727 - KOG0254 Predicted transporter (major facilitator superfamily), at 536,738 to 538,804 13727 mRNA_5360: 13728 - HMMPfam-Xylose isomerase-like TIM barrel-PF01261,SUPERFAMILY--SSF51658, at 539,342 to 540,520 13728 mRNA_5361: 13729 - K00511 SQLE, ERG1 squalene monooxygenase, at 541,060 to 542,859 13729
Group Condition mRNA_5357 mRNA_5358 mRNA_5359 mRNA_5360 mRNA_5361
13725 13726 13727 13728 13729
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 N.D. -0.3 +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 N.D. -0.8 -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. -0.8 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -0.6 +0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.0 -0.1 N.D.
auxotrophy Dropout Complete +0.1 N.D. -0.3 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. -0.1 +0.1 N.D.
auxotrophy YNB Glucose -0.3 N.D. -0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. +0.0 -0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.1 -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. +0.4 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.3 +0.1 N.D.
growth on oleic acid YNB Glucose +0.1 N.D. +0.4 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 N.D. +0.4 -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. +0.2 +0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. +0.4 +0.3 N.D.
growth on ypd YPD +0.5 N.D. +0.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. +0.5 -0.2 N.D.
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.6 +0.3 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +1.7 -0.6 N.D.
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