Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13736 and 13737 are separated by 518 nucleotides13737 and 13738 are separated by 524 nucleotides13738 and 13739 are separated by 313 nucleotides13739 and 13740 are separated by 416 nucleotides mRNA_5368: 13736 - K09553 STIP1 stress-induced-phosphoprotein 1, at 555,613 to 556,991 13736 mRNA_5369: 13737 - K03627 MBF1 putative transcription factor, at 557,510 to 559,067 13737 mRNA_5370: 13738 - K18443 GBF1 golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, at 559,592 to 565,388 13738 mRNA_5371: 13739 - K03859 PIGC, GPI2 phosphatidylinositol glycan, class C, at 565,702 to 566,965 13739 mRNA_5372: 13740 - K04487 iscS, NFS1 cysteine desulfurase, at 567,382 to 569,979 13740
Group Condition mRNA_5368 mRNA_5369 mRNA_5370 mRNA_5371 mRNA_5372
13736 13737 13738 13739 13740
ypd buoyancy 40 hrs No Buoyancy Separation -0.4 +0.1 -2.0 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.0 -1.4 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.2 -0.5 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.7 -0.2 N.D. N.D.
growth on oleic acid YNB Glucose +0.1 -0.5 -0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 -0.2 -0.7 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.3 -0.3 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.3 -0.3 N.D. N.D.
growth on ypd YPD +0.3 +0.0 +0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.1 +0.0 +0.5 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +1.7 -0.7 N.D. N.D.
auxotrophy Dropout Complete +0.3 +1.5 -0.5 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.1 +1.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.2 +2.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 +1.6 +0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 +1.9 +0.5 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.0 +0.9 +1.3 N.D. N.D.
auxotrophy YNB Glucose +0.7 +1.2 +0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.7 +2.3 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +1.3 +1.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.6 +3.1 N.D. N.D.
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