Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13737 and 13738 are separated by 524 nucleotides13738 and 13739 are separated by 313 nucleotides13739 and 13740 are separated by 416 nucleotides13740 and 13741 are separated by 1083 nucleotides mRNA_5369: 13737 - K03627 MBF1 putative transcription factor, at 557,510 to 559,067 13737 mRNA_5370: 13738 - K18443 GBF1 golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, at 559,592 to 565,388 13738 mRNA_5371: 13739 - K03859 PIGC, GPI2 phosphatidylinositol glycan, class C, at 565,702 to 566,965 13739 mRNA_5372: 13740 - K04487 iscS, NFS1 cysteine desulfurase, at 567,382 to 569,979 13740 mRNA_5373: 13741 - Hypothetical Protein, at 571,063 to 571,885 13741
Group Condition mRNA_5369 mRNA_5370 mRNA_5371 mRNA_5372 mRNA_5373
13737 13738 13739 13740 13741
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -1.4 N.D. N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -2.0 N.D. N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.5 N.D. N.D. -0.7
growth on oleic acid YNB Glucose -0.5 -0.2 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.7 N.D. N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.7 -0.2 N.D. N.D. +0.3
growth on ypd YPD +0.0 +0.1 N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid +0.0 +0.5 N.D. N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.3 N.D. N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.3 N.D. N.D. +0.2
auxotrophy Dropout Complete +1.5 -0.5 N.D. N.D. -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +1.1 N.D. N.D. -0.3
auxotrophy YNB Glucose +1.2 +0.5 N.D. N.D. -0.4
ypd buoyancy 40 hrs Median Buoyancy +1.7 -0.7 N.D. N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine +2.3 -0.1 N.D. N.D. -0.4
growth on ricinoleic acid YNB Glucose +0.9 +1.3 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine +1.9 +0.5 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +2.2 N.D. N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +1.6 +0.5 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.3 +1.6 N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +3.1 N.D. N.D. -0.1
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