Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13738 and 13739 are separated by 313 nucleotides13739 and 13740 are separated by 416 nucleotides13740 and 13741 are separated by 1083 nucleotides13741 and 13742 are separated by 74 nucleotides mRNA_5370: 13738 - K18443 GBF1 golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, at 559,592 to 565,388 13738 mRNA_5371: 13739 - K03859 PIGC, GPI2 phosphatidylinositol glycan, class C, at 565,702 to 566,965 13739 mRNA_5372: 13740 - K04487 iscS, NFS1 cysteine desulfurase, at 567,382 to 569,979 13740 mRNA_5373: 13741 - Hypothetical Protein, at 571,063 to 571,885 13741 mRNA_5374: 13742 - KOG0001 Ubiquitin and ubiquitin-like proteins, at 571,960 to 573,012 13742
Group Condition mRNA_5370 mRNA_5371 mRNA_5372 mRNA_5373 mRNA_5374
13738 13739 13740 13741 13742
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. N.D. -0.7 -0.8
ypd buoyancy 40 hrs No Buoyancy Separation -2.0 N.D. N.D. +0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -1.4 N.D. N.D. -0.2 +0.1
growth on oleic acid YNB Glucose -0.2 N.D. N.D. -0.4 -0.7
auxotrophy Dropout Complete -0.5 N.D. N.D. -0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.1 N.D. N.D. -0.4 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.7 N.D. N.D. +0.4 -0.1
growth on oleic acid YNB Oleic Acid +0.5 N.D. N.D. -0.4 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. N.D. +0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. N.D. +0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 N.D. N.D. +0.3 +0.3
auxotrophy YNB Glucose +0.5 N.D. N.D. -0.4 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +1.1 N.D. N.D. -0.3 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. N.D. +0.3 +0.2
growth on ypd YPD +0.1 N.D. N.D. -0.2 +0.5
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. N.D. -0.2 +0.2
growth on ricinoleic acid YNB Glucose +1.3 N.D. N.D. -0.3 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.5 N.D. N.D. +0.4 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.6 N.D. N.D. +0.1 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +2.2 N.D. N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +3.1 N.D. N.D. -0.1 +0.2
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