Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13739 and 13740 are separated by 416 nucleotides13740 and 13741 are separated by 1083 nucleotides13741 and 13742 are separated by 74 nucleotides13742 and 13743 are separated by 5336 nucleotides mRNA_5371: 13739 - K03859 PIGC, GPI2 phosphatidylinositol glycan, class C, at 565,702 to 566,965 13739 mRNA_5372: 13740 - K04487 iscS, NFS1 cysteine desulfurase, at 567,382 to 569,979 13740 mRNA_5373: 13741 - Hypothetical Protein, at 571,063 to 571,885 13741 mRNA_5374: 13742 - KOG0001 Ubiquitin and ubiquitin-like proteins, at 571,960 to 573,012 13742 mRNA_5375: 13743 - Hypothetical Protein, at 578,349 to 578,853 13743
Group Condition mRNA_5371 mRNA_5372 mRNA_5373 mRNA_5374 mRNA_5375
13739 13740 13741 13742 13743
auxotrophy Dropout Complete N.D. N.D. -0.4 -0.2 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.7 -0.8 +0.6
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.4 -0.1 -0.3
growth on oleic acid YNB Glucose N.D. N.D. -0.4 -0.7 +0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.4 -0.4 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.3 -0.5 +0.1
auxotrophy YNB Glucose N.D. N.D. -0.4 -0.0 -0.1
growth on ypd YPD N.D. N.D. -0.2 +0.5 -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 -0.4 +0.5
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.2 +0.2 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 -0.0 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.1 +0.2 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.2 +0.1 +0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.4 -0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.4 -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 -0.0 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.3 +0.3 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 +0.2 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.3 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.5 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.3 +0.1 +1.0
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