Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13745 and 13746 are separated by 2989 nucleotides13746 and 13747 are separated by 201 nucleotides13747 and 13748 are separated by 124 nucleotides13748 and 13749 are separated by 255 nucleotides mRNA_5377: 13745 - Hypothetical Protein, at 587,217 to 590,101 13745 mRNA_5378: 13746 - Hypothetical Protein, at 593,091 to 593,885 13746 mRNA_5379: 13747 - K12829 SF3B2, SAP145, CUS1 splicing factor 3B subunit 2, at 594,087 to 596,220 13747 mRNA_5380: 13748 - K17782 MIA40, CHCHD4 mitochondrial intermembrane space import and assembly protein 40, at 596,345 to 597,306 13748 mRNA_5381: 13749 - Hypothetical Protein, at 597,562 to 598,053 13749
Group Condition mRNA_5377 mRNA_5378 mRNA_5379 mRNA_5380 mRNA_5381
13745 13746 13747 13748 13749
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.5 N.D. N.D. -0.9
facs on lipid accumulation Sorted No BODIPY Gate -0.0 -0.2 N.D. N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.4 N.D. N.D. -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.4 N.D. N.D. -0.5
growth on ricinoleic acid YNB Glucose -0.0 +0.4 N.D. N.D. -0.3
growth on oleic acid YNB Oleic Acid -0.1 +0.5 N.D. N.D. -0.4
auxotrophy YNB Glucose +0.3 +0.3 N.D. N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.2 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.3 N.D. N.D. -0.4
growth on oleic acid YNB Glucose +0.1 +0.3 N.D. N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 N.D. N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.0 N.D. N.D. +0.7
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 +0.6 N.D. N.D. +0.1
auxotrophy Dropout Complete +0.2 +0.3 N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine +0.4 +0.4 N.D. N.D. -0.1
growth on ypd YPD +0.1 +0.3 N.D. N.D. +0.6
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.3 N.D. N.D. +0.9
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.9 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.6 N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.7 N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +1.1 N.D. N.D. +0.4
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