Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13754 and 13755 are separated by 2466 nucleotides13755 and 13756 are separated by 3186 nucleotides13756 and 13757 are separated by 150 nucleotides13757 and 13758 are separated by 547 nucleotides mRNA_5386: 13754 - Hypothetical Protein, at 620,472 to 621,845 13754 mRNA_5387: 13755 - Hypothetical Protein, at 624,312 to 624,933 13755 mRNA_5388: 13756 - K02931 RP-L5, MRPL5, rplE large subunit ribosomal protein L5, at 628,120 to 629,112 13756 mRNA_5389: 13757 - KOG0750 Mitochondrial solute carrier protein, at 629,263 to 631,005 13757 mRNA_5390: 13758 - K03065 PSMC3, RPT5 26S proteasome regulatory subunit T5, at 631,553 to 634,043 13758
Group Condition mRNA_5386 mRNA_5387 mRNA_5388 mRNA_5389 mRNA_5390
13754 13755 13756 13757 13758
facs on lipid accumulation Sorted High BODIPY Gate -1.4 +0.2 N.D. -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.3 N.D. -0.9 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.0 N.D. -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.2 N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.4 N.D. -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Glucose +1.0 -0.4 N.D. -0.8 N.D.
growth on oleic acid YNB Glucose +0.5 -0.1 N.D. -0.6 N.D.
growth on ypd YPD +0.1 -0.5 N.D. +0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.1 +0.2 N.D. -0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.1 N.D. -0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +1.2 -0.6 N.D. -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 +0.6 N.D. -0.8 N.D.
auxotrophy YNB Glucose +1.1 -0.2 N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 +0.3 N.D. -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 +0.1 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 -0.1 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +1.3 +0.5 N.D. -0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +1.6 +0.2 N.D. +0.1 N.D.
auxotrophy Dropout Complete +2.2 -0.1 N.D. +0.1 N.D.
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