Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13757 and 13758 are separated by 547 nucleotides13758 and 13759 are separated by 344 nucleotides13759 and 13760 are separated by 1716 nucleotides13760 and 13761 are separated by 1577 nucleotides mRNA_5389: 13757 - KOG0750 Mitochondrial solute carrier protein, at 629,263 to 631,005 13757 mRNA_5390: 13758 - K03065 PSMC3, RPT5 26S proteasome regulatory subunit T5, at 631,553 to 634,043 13758 mRNA_5391: 13759 - HMMPfam-ATP synthase j chain-PF04911, at 634,388 to 634,888 13759 mRNA_5392: 13760 - KOG0166 Karyopherin (importin) alpha, at 636,605 to 638,927 13760 mRNA_5393: 13761 - KOG0603 Ribosomal protein S6 kinase, at 640,505 to 641,434 13761
Group Condition mRNA_5389 mRNA_5390 mRNA_5391 mRNA_5392 mRNA_5393
13757 13758 13759 13760 13761
facs on lipid accumulation Sorted No BODIPY Gate -0.9 N.D. N.D. N.D. -0.1
growth on ricinoleic acid YNB Glucose -0.8 N.D. N.D. N.D. -0.0
growth on oleic acid YNB Glucose -0.6 N.D. N.D. N.D. -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. N.D. N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.3 N.D. N.D. N.D. -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. N.D. N.D. +0.0
auxotrophy YNB Glucose -0.5 N.D. N.D. N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 N.D. N.D. N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 N.D. N.D. N.D. +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. N.D. N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. N.D. +0.1
auxotrophy Dropout Complete +0.1 N.D. N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. N.D. N.D. +0.2
growth on ypd YPD +0.4 N.D. N.D. N.D. -0.0
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