Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13770 and 13771 are separated by 283 nucleotides13771 and 13772 are separated by 897 nucleotides13772 and 13773 are separated by 119 nucleotides13773 and 13774 are separated by 145 nucleotides mRNA_5402: 13770 - KOG2938 Predicted inosine-uridine preferring nucleoside hydrolase, at 17,110 to 18,696 13770 mRNA_5403: 13771 - K00789 metK S-adenosylmethionine synthetase, at 18,980 to 21,610 13771 mRNA_5404: 13772 - Hypothetical Protein, at 22,508 to 23,850 13772 mRNA_5405: 13773 - K08869 ADCK, ABC1 aarF domain-containing kinase, at 23,970 to 26,723 13773 mRNA_5406: 13774 - KOG2742 Predicted oxidoreductase, at 26,869 to 28,382 13774
Group Condition mRNA_5402 mRNA_5403 mRNA_5404 mRNA_5405 mRNA_5406
13770 13771 13772 13773 13774
auxotrophy YNB Glucose -0.5 N.D. +0.2 -0.4 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 N.D. +0.0 -0.1 -0.1
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. +0.0 -0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. +0.0 -0.2 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.0 +0.0 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.2 -0.2 -0.1
growth on oleic acid YNB Oleic Acid -0.0 N.D. +0.1 -0.2 +0.2
auxotrophy Dropout Complete +0.2 N.D. +0.1 -0.2 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 N.D. +0.4 -0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.6 -0.3 -0.3
growth on ricinoleic acid YNB Glucose -0.0 N.D. +0.7 -0.3 -0.1
growth on oleic acid YNB Glucose -0.1 N.D. +0.2 -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.0 N.D. -0.1 +0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. +0.6 -0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.1 +0.2 +0.3
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.0 +0.2 +0.0
growth on ypd YPD +0.3 N.D. -0.0 +0.4 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.2 +0.6 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. +0.3 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. +0.3 +0.4 +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. +0.3 +0.6 +0.7
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