Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13775 and 13776 are separated by 275 nucleotides13776 and 13777 are separated by 1240 nucleotides13777 and 13778 are separated by 993 nucleotides13778 and 13779 are separated by 545 nucleotides mRNA_5407: 13775 - K15734 SDR16C5 all-trans-retinol dehydrogenase (NAD+), at 28,474 to 30,165 13775 mRNA_5408: 13776 - HMMPfam-Protein kinase domain-PF00069,ProSitePatterns-Tyrosine protein kinases specific active-site signature.-PS00109,ProSiteProfiles-Protein kinase domain profile.-PS50011,SUPERFAMILY--SSF56112, at 30,441 to 31,506 13776 mRNA_5409: 13777 - Hypothetical Protein, at 32,747 to 36,067 13777 mRNA_5410: 13778 - KOG2952 Cell cycle control protein, at 37,061 to 38,634 13778 mRNA_5411: 13779 - K03251 EIF3D translation initiation factor 3 subunit D, at 39,180 to 41,133 13779
Group Condition mRNA_5407 mRNA_5408 mRNA_5409 mRNA_5410 mRNA_5411
13775 13776 13777 13778 13779
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 -0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.0 N.D. N.D.
auxotrophy Dropout Complete N.D. -0.3 +0.0 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 -0.2 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.0 N.D. N.D.
auxotrophy YNB Glucose N.D. -0.1 +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 -0.1 N.D. N.D.
growth on ypd YPD N.D. -0.0 +0.1 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 +0.0 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.2 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 -0.2 N.D. N.D.
growth on oleic acid YNB Glucose N.D. +0.3 +0.1 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 +0.4 N.D. N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.7 +0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.4 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.5 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.5 +0.5 N.D. N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.5 +0.4 N.D. N.D.
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