Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13783 and 13784 are separated by 1849 nucleotides13784 and 13785 are separated by 145 nucleotides13785 and 13786 are separated by 193 nucleotides13786 and 13787 are separated by 305 nucleotides mRNA_5415: 13783 - HMMPfam-Tyrosyl-DNA phosphodiesterase-PF06087,SUPERFAMILY--SSF56024, at 48,943 to 50,652 13783 mRNA_5416: 13784 - K04496 CTBP C-terminal binding protein, at 52,502 to 53,852 13784 mRNA_5417: 13785 - ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 53,998 to 56,725 13785 mRNA_5418: 13786 - K01502 E3.5.5.7 aliphatic nitrilase, at 56,919 to 58,432 13786 mRNA_5419: 13787 - Interpro Cut8, nuclear proteasome tether protein, at 58,738 to 60,106 13787
Group Condition mRNA_5415 mRNA_5416 mRNA_5417 mRNA_5418 mRNA_5419
13783 13784 13785 13786 13787
growth on ypd YPD -0.5 -0.2 -0.1 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.2 +0.1 +0.0 +0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.0 +0.1 -0.1 -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 +0.1 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.0 +0.0 +0.1 -0.1
auxotrophy YNB Glucose +0.1 +0.1 -0.4 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.1 +0.2 -0.0 +0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.4 +0.0 -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 -0.1 +0.0 +0.4
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 +0.1 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 -0.4 +0.2 +0.5
auxotrophy Dropout Complete +0.1 +0.0 -0.1 +0.1 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.9 +0.4 -0.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.1 +0.1 +0.4
growth on ricinoleic acid YNB Glucose +0.2 +0.1 +0.1 +0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.2 -0.2 +0.0 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.0 +0.1 +0.1 +0.1 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.0 +0.2 +0.3 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.6 +0.2 +0.1 +0.1
growth on oleic acid YNB Oleic Acid +0.2 +0.2 +0.0 +0.1 +1.0
growth on oleic acid YNB Glucose +0.3 +0.3 -0.1 +0.1 +0.9
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