Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13785 and 13786 are separated by 193 nucleotides13786 and 13787 are separated by 305 nucleotides13787 and 13788 are separated by 350 nucleotides13788 and 13789 are separated by 449 nucleotides mRNA_5417: 13785 - ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 53,998 to 56,725 13785 mRNA_5418: 13786 - K01502 E3.5.5.7 aliphatic nitrilase, at 56,919 to 58,432 13786 mRNA_5419: 13787 - Interpro Cut8, nuclear proteasome tether protein, at 58,738 to 60,106 13787 mRNA_5420: 13788 - Hypothetical Protein, at 60,457 to 62,427 13788 mRNA_5421: 13789 - Interpro Domain of unknown function (DUF5102) 0, at 62,877 to 64,571 13789
Group Condition mRNA_5417 mRNA_5418 mRNA_5419 mRNA_5420 mRNA_5421
13785 13786 13787 13788 13789
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 -0.2 -0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.4 -0.3 -0.2 +0.1 -0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.1 -0.0 +0.0 -0.1
growth on ypd YPD -0.1 -0.0 +0.2 +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 +0.2 -0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.1 +0.1 +0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.0 -0.0 +0.2 +0.2 -0.4
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.0 +0.5 +0.0 -0.1
auxotrophy YNB Glucose -0.4 +0.1 +0.3 -0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine -0.4 +0.2 +0.5 -0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.0 +0.4 +0.1 +0.4
auxotrophy Dropout Complete -0.1 +0.1 +0.5 -0.2 +0.7
growth on oleic acid YNB Glucose -0.1 +0.1 +0.9 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 +0.1 -0.1 +0.4 +0.7
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.1 +0.6 -0.2 +0.7
growth on oleic acid YNB Oleic Acid +0.0 +0.1 +1.0 +0.2 +0.2
growth on ricinoleic acid YNB Glucose +0.1 +0.3 +0.3 -0.1 +1.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.0 +0.4 +0.2 +1.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.1 +0.4 +0.0 +1.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.0 +0.4 +0.4 +1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.3 +0.5 +0.4 +1.2
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