Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13799 and 13800 are separated by 704 nucleotides13800 and 13801 are separated by 642 nucleotides13801 and 13802 are separated by 194 nucleotides13802 and 13803 are separated by 117 nucleotides mRNA_5431: 13799 - KOG1296 Uncharacterized conserved protein, at 84,428 to 85,305 13799 mRNA_5432: 13800 - Hypothetical Protein, at 86,010 to 86,874 13800 mRNA_5433: 13801 - BLAST UPF0658 Golgi apparatus membrane protein [Termitomyce..., at 87,517 to 89,164 13801 mRNA_5434: 13802 - KOG2852 Possible oxidoreductase, at 89,359 to 90,914 13802 mRNA_5435: 13803 - Hypothetical Protein, at 91,032 to 92,817 13803
Group Condition mRNA_5431 mRNA_5432 mRNA_5433 mRNA_5434 mRNA_5435
13799 13800 13801 13802 13803
facs on lipid accumulation Sorted High BODIPY Gate -0.5 -0.4 -0.1 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.3 -0.2 -0.2 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.9 -0.1 +0.2 +0.1
auxotrophy Dropout Complete +0.1 -0.6 -0.1 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.2 -0.2 -0.4 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.2 -0.1 +0.0 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 -0.3 -0.2 +0.1
auxotrophy YNB Glucose +0.2 -0.1 -0.1 -0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.0 +0.1 +0.3 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.1 -0.2 +0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 -0.6 -0.2 -0.0 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 -0.1 -0.0 +0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.2 -0.1 -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.3 +0.1 +0.1 +0.3
growth on ricinoleic acid YNB Glucose -0.1 +0.3 -0.2 +0.2 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.4 +0.1 +0.0 +0.0
growth on oleic acid YNB Oleic Acid +0.1 -0.2 +0.2 +0.3 +0.3
growth on oleic acid YNB Glucose +0.3 -0.2 +0.0 +0.3 +0.3
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.6 +0.1 +0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.8 -0.2 +0.1 +0.5
growth on ypd YPD +0.2 +1.0 -0.3 +0.5 -0.0
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