Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13810 and 13811 are separated by 144 nucleotides13811 and 13812 are separated by 188 nucleotides13812 and 13813 are separated by 1088 nucleotides13813 and 13814 are separated by 649 nucleotides mRNA_5442: 13810 - HMMPfam-R3H domain-PF01424,HMMPfam-SUZ domain-PF12752,ProSiteProfiles-R3H domain profile.-PS51061,ProSiteProfiles-SUZ domain profile.-PS51673,SUPERFAMILY--SSF82708, at 103,873 to 106,982 13810 mRNA_5443: 13811 - KOG3085 Predicted hydrolase (HAD superfamily), at 107,127 to 108,436 13811 mRNA_5444: 13812 - HMMPfam-Eukaryotic cytochrome b561-PF03188,ProSiteProfiles-Cytochrome b561 domain profile.-PS50939,SMART-Cytochrome b-561 / ferric reductase transmembrane domain.-SM00665, at 108,625 to 109,612 13812 mRNA_5445: 13813 - K07513 ACAA1 acetyl-CoA acyltransferase 1, at 110,701 to 112,520 13813 mRNA_5446: 13814 - HMMPfam-Stress responsive A/B Barrel Domain-PF07876,ProSiteProfiles-Stress-response A/B barrel domain profile.-PS51502,SMART-Stress responsive A/B Barrel Domain-SM00886,SUPERFAMILY--SSF54909, at 113,170 to 113,689 13814
Group Condition mRNA_5442 mRNA_5443 mRNA_5444 mRNA_5445 mRNA_5446
13810 13811 13812 13813 13814
growth on ricinoleic acid YNB Ricinoleic Acid -1.4 -0.1 +0.6 -1.7 N.D.
growth on oleic acid YNB Oleic Acid -0.5 +0.2 +0.2 -2.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -2.3 -0.3 +0.4 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.8 -0.2 +0.4 -1.6 N.D.
auxotrophy Dropout Complete -1.8 -0.1 +0.1 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.9 +0.1 +0.0 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.3 -0.0 -0.1 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -1.2 +0.2 +0.1 -0.4 N.D.
auxotrophy YNB Glucose -1.0 +0.0 -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.7 -0.1 +0.3 -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -1.3 -0.2 +0.6 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.1 -0.4 -0.2 N.D.
growth on ypd YPD -0.6 -0.2 -0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.1 -0.2 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 +0.1 -0.3 -0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.4 -0.1 +0.5 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.2 -0.1 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.5 +0.3 +0.2 -0.2 N.D.
growth on oleic acid YNB Glucose -0.2 +0.1 +0.3 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.2 +0.1 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +1.3 +0.4 +0.1 -0.2 N.D.
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