Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13820 and 13821 are separated by 787 nucleotides13821 and 13822 are separated by 166 nucleotides13822 and 13823 are separated by 63 nucleotides13823 and 13824 are separated by 978 nucleotides mRNA_5452: 13820 - HMMPfam-Eukaryotic aspartyl protease-PF00026,PRINTS-Pepsin (A1) aspartic protease family signature-PR00792,ProSitePatterns-Eukaryotic and viral aspartyl proteases active site.-PS00141,SUPERFAMILY--SSF50630, at 124,272 to 125,754 13820 mRNA_5453: 13821 - HMMPfam-Glutaredoxin-like domain (DUF836)-PF05768,SUPERFAMILY--SSF52833, at 126,542 to 126,978 13821 mRNA_5454: 13822 - KOG2673 Uncharacterized conserved protein, contains PSP domain, at 127,145 to 127,753 13822 mRNA_5455: 13823 - PRINTS-Proline rich extensin signature-PR01217, at 127,817 to 128,320 13823 mRNA_5456: 13824 - K07034 K07034 uncharacterized protein, at 129,299 to 130,839 13824
Group Condition mRNA_5452 mRNA_5453 mRNA_5454 mRNA_5455 mRNA_5456
13820 13821 13822 13823 13824
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. +0.3 N.D. -1.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -1.0 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -1.1 N.D. +0.3
growth on ricinoleic acid YNB Glucose +0.4 N.D. -0.4 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 N.D. -0.7 N.D. +0.4
growth on ypd YPD +0.1 N.D. +0.1 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.3 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. +0.1 N.D. -0.5
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. -0.1 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. -0.5 N.D. +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.2 N.D. +0.0
growth on oleic acid YNB Oleic Acid +0.3 N.D. -0.0 N.D. -0.2
growth on oleic acid YNB Glucose +0.2 N.D. +0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.3 N.D. +0.6
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 N.D. -0.2 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -0.6 N.D. +1.2
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.5 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. +0.3 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.4 N.D. +0.2
auxotrophy Dropout Complete +0.4 N.D. +0.7 N.D. -0.1
auxotrophy YNB Glucose +0.5 N.D. +0.9 N.D. +0.2
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