Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13830 and 13831 are separated by 550 nucleotides13831 and 13832 are separated by 953 nucleotides13832 and 13833 are separated by 407 nucleotides13833 and 13834 are separated by 203 nucleotides mRNA_5462: 13830 - HMMPfam-Bacteriorhodopsin-like protein-PF01036,PRINTS-Bacterial opsin signature-PR00251,ProSitePatterns-Bacterial rhodopsins signature 1.-PS00950,SMART-Bacteriorhodopsin-like protein-SM01021,SUPERFAMILY--SSF81321, at 148,438 to 149,863 13830 mRNA_5463: 13831 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 150,414 to 154,047 13831 mRNA_5464: 13832 - K09579 PIN4 peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, at 155,001 to 155,781 13832 mRNA_5465: 13833 - Hypothetical Protein, at 156,189 to 157,604 13833 mRNA_5466: 13834 - K05841 E2.4.1.173 sterol 3beta-glucosyltransferase, at 157,808 to 163,476 13834
Group Condition mRNA_5462 mRNA_5463 mRNA_5464 mRNA_5465 mRNA_5466
13830 13831 13832 13833 13834
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.0 N.D. -0.5 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.3 N.D. -0.3 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -0.1 N.D. -0.1 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 N.D. -0.3 +0.2
auxotrophy Dropout Complete -0.0 -0.1 N.D. +0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.2 N.D. +0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.3 N.D. +0.5 -0.9
auxotrophy YNB Glucose +0.3 -0.3 N.D. +0.1 -0.0
growth on ricinoleic acid YNB Glucose +0.3 -0.2 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.4 N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 N.D. +0.6 -0.5
growth on ypd YPD +0.1 +0.0 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 +0.2 N.D. +0.4 +0.1
growth on oleic acid YNB Glucose +0.1 -0.0 N.D. +0.5 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.1 N.D. +0.5 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.2 N.D. -0.0 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.4 N.D. +0.6 -0.4
growth on oleic acid YNB Oleic Acid +0.1 +0.0 N.D. +0.9 -0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 N.D. +0.8 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 N.D. +0.8 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 -0.1 N.D. +1.4 +0.2
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