Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13831 and 13832 are separated by 953 nucleotides13832 and 13833 are separated by 407 nucleotides13833 and 13834 are separated by 203 nucleotides13834 and 13835 are separated by 1639 nucleotides mRNA_5463: 13831 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 150,414 to 154,047 13831 mRNA_5464: 13832 - K09579 PIN4 peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, at 155,001 to 155,781 13832 mRNA_5465: 13833 - Hypothetical Protein, at 156,189 to 157,604 13833 mRNA_5466: 13834 - K05841 E2.4.1.173 sterol 3beta-glucosyltransferase, at 157,808 to 163,476 13834 mRNA_5467: 13835 - KOG4391 Predicted alpha/beta hydrolase BEM46, at 165,116 to 166,451 13835
Group Condition mRNA_5463 mRNA_5464 mRNA_5465 mRNA_5466 mRNA_5467
13831 13832 13833 13834 13835
auxotrophy Dropout Complete -0.1 N.D. +0.0 -0.2 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +0.5 -0.9 -0.4
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.3 +0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. -0.1 -0.0 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. -0.5 -0.2 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 N.D. +0.1 -0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. -0.3 +0.2 +0.2
auxotrophy YNB Glucose -0.3 N.D. +0.1 -0.0 +0.2
growth on ypd YPD +0.0 N.D. -0.0 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.0 +0.4 -0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. +0.1 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. +0.6 -0.4 -0.3
growth on ricinoleic acid YNB Glucose -0.2 N.D. +0.1 -0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.6 -0.5 +0.0
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. +0.5 -0.1 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.8 +0.0 -0.4
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.9 -0.0 -0.3
growth on oleic acid YNB Glucose -0.0 N.D. +0.5 +0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.4 +0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.8 +0.0 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. +1.4 +0.2 +0.1
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