Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13840 and 13841 are separated by 137 nucleotides13841 and 13842 are separated by 278 nucleotides13842 and 13843 are separated by 158 nucleotides13843 and 13844 are separated by 438 nucleotides mRNA_5472: 13840 - K00728 POMT dolichyl-phosphate-mannose-protein mannosyltransferase, at 173,167 to 176,789 13840 mRNA_5473: 13841 - KOG2226 Proteins containing regions of low-complexity, at 176,927 to 180,681 13841 mRNA_5474: 13842 - HMMPfam-Mitochondrial ATP synthase epsilon chain-PF04627,SUPERFAMILY--SSF48690, at 180,960 to 181,593 13842 mRNA_5475: 13843 - KOG0084 GTPase Rab1/YPT1, small G protein superfamily, and related GTP-binding proteins, at 181,752 to 183,361 13843 mRNA_5476: 13844 - KOG4719 Nuclear pore complex protein, at 183,800 to 188,314 13844
Group Condition mRNA_5472 mRNA_5473 mRNA_5474 mRNA_5475 mRNA_5476
13840 13841 13842 13843 13844
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 N.D. -0.4 -1.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. -0.0 -1.7
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 N.D. -0.3 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.4 N.D. -0.7 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 N.D. +0.1 -1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.6 N.D. -0.3 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. -0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.0 N.D. +0.5 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 N.D. -0.3 +0.1
growth on ypd YPD N.D. +0.4 N.D. -0.1 -0.7
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. -0.1 -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.0 N.D. -0.4 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.0 N.D. -0.5 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 N.D. -0.1 -0.2
auxotrophy YNB Glucose N.D. +0.2 N.D. -0.2 -0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 N.D. -0.2 -0.2
auxotrophy Dropout Complete N.D. +0.2 N.D. -0.4 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 N.D. -0.4 +0.2
growth on oleic acid YNB Oleic Acid N.D. -0.4 N.D. -0.3 +1.3
growth on oleic acid YNB Glucose N.D. -0.1 N.D. -0.0 +1.2
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