Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13858 and 13859 are separated by 353 nucleotides13859 and 13860 are separated by 1130 nucleotides13860 and 13861 are separated by 429 nucleotides13861 and 13862 are separated by 244 nucleotides mRNA_5490: 13858 - Hypothetical Protein, at 219,614 to 220,900 13858 mRNA_5491: 13859 - Hypothetical Protein, at 221,254 to 222,129 13859 mRNA_5492: 13860 - K15436 TRPO3, MTR10 transportin-3, at 223,260 to 226,500 13860 mRNA_5493: 13861 - KOG0297 TNF receptor-associated factor, at 226,930 to 227,786 13861 mRNA_5494: 13862 - KOG1119 Mitochondrial Fe-S cluster biosynthesis protein ISA2 (contains a HesB-like domain), at 228,031 to 229,041 13862
Group Condition mRNA_5490 mRNA_5491 mRNA_5492 mRNA_5493 mRNA_5494
13858 13859 13860 13861 13862
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.6 N.D. -0.7 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 -1.3 N.D. -0.0 N.D.
growth on ypd YPD -0.1 +0.1 N.D. -0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 -0.2 N.D. -0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 +0.6 N.D. -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.5 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.5 N.D. -0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 +0.8 N.D. -0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 +0.5 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Glucose -0.1 +0.2 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +0.7 N.D. -0.4 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.2 N.D. -0.0 N.D.
auxotrophy Dropout Complete -0.0 +0.5 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.4 -0.2 N.D. +0.2 N.D.
auxotrophy YNB Glucose +0.2 +0.2 N.D. +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.4 +0.2 N.D. -0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.0 +0.6 N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.1 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.6 +0.1 N.D. +0.0 N.D.
growth on oleic acid YNB Glucose -0.1 +0.7 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.7 N.D. -0.1 N.D.
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