Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13859 and 13860 are separated by 1130 nucleotides13860 and 13861 are separated by 429 nucleotides13861 and 13862 are separated by 244 nucleotides13862 and 13863 are separated by 270 nucleotides mRNA_5491: 13859 - Hypothetical Protein, at 221,254 to 222,129 13859 mRNA_5492: 13860 - K15436 TRPO3, MTR10 transportin-3, at 223,260 to 226,500 13860 mRNA_5493: 13861 - KOG0297 TNF receptor-associated factor, at 226,930 to 227,786 13861 mRNA_5494: 13862 - KOG1119 Mitochondrial Fe-S cluster biosynthesis protein ISA2 (contains a HesB-like domain), at 228,031 to 229,041 13862 mRNA_5495: 13863 - K10733 GINS2, PSF2 GINS complex subunit 2, at 229,312 to 230,460 13863
Group Condition mRNA_5491 mRNA_5492 mRNA_5493 mRNA_5494 mRNA_5495
13859 13860 13861 13862 13863
facs on lipid accumulation Sorted High BODIPY Gate -0.6 N.D. -0.7 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 N.D. -0.0 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. -0.7 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.5 N.D. -0.1 N.D. N.D.
growth on ypd YPD +0.1 N.D. -0.5 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.2 N.D. +0.2 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.1 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.0 N.D. N.D.
growth on ricinoleic acid YNB Glucose +0.2 N.D. -0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. -0.3 N.D. N.D.
auxotrophy YNB Glucose +0.2 N.D. +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. +0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. -0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.7 N.D. -0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.8 N.D. -0.5 N.D. N.D.
auxotrophy Dropout Complete +0.5 N.D. -0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. +0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.6 N.D. +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine +0.7 N.D. -0.1 N.D. N.D.
growth on oleic acid YNB Glucose +0.7 N.D. +0.1 N.D. N.D.
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