Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 13860 and 13861 are separated by 429 nucleotides13861 and 13862 are separated by 244 nucleotides13862 and 13863 are separated by 270 nucleotides13863 and 13864 are separated by 455 nucleotides mRNA_5492: 13860 - K15436 TRPO3, MTR10 transportin-3, at 223,260 to 226,500 13860 mRNA_5493: 13861 - KOG0297 TNF receptor-associated factor, at 226,930 to 227,786 13861 mRNA_5494: 13862 - KOG1119 Mitochondrial Fe-S cluster biosynthesis protein ISA2 (contains a HesB-like domain), at 228,031 to 229,041 13862 mRNA_5495: 13863 - K10733 GINS2, PSF2 GINS complex subunit 2, at 229,312 to 230,460 13863 mRNA_5496: 13864 - HMMPfam-HSF-type DNA-binding-PF00447,SUPERFAMILY--SSF46785, at 230,916 to 233,624 13864
Group Condition mRNA_5492 mRNA_5493 mRNA_5494 mRNA_5495 mRNA_5496
13860 13861 13862 13863 13864
growth on ypd YPD N.D. -0.5 N.D. N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.7 N.D. N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 N.D. N.D. -0.7
auxotrophy YNB Glucose N.D. +0.0 N.D. N.D. -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.5 N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 N.D. N.D. -0.2
growth on ricinoleic acid YNB Glucose N.D. -0.1 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 N.D. N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. N.D. +0.0
auxotrophy Dropout Complete N.D. -0.1 N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.4 N.D. N.D. +0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 N.D. N.D. +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.7 N.D. N.D. +1.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.1 N.D. N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.3 N.D. N.D. +1.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 N.D. N.D. +0.9
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.2 N.D. N.D. +0.8
growth on oleic acid YNB Oleic Acid N.D. +0.0 N.D. N.D. +1.3
growth on oleic acid YNB Glucose N.D. +0.1 N.D. N.D. +1.4
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